Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells

1 January 1986

journal article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 14 (20) , 7823-7838
https://doi.org/10.1093/nar/14.20.7823

Abstract

We have isolated, cloned and analyzed small polydisperse circular (spc) DNA from mouse 3T6 cells. The representation of highly repeated mouse genome sequence families in spcDNA has been examined, and the B1 repeat appears overrepresented in spcDNA by two criteria. The majority of spcDNA clones, however, is made out by as yet uncharacterized middle repetitive sequences. We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.

Keywords

This publication has 32 references indexed in Scilit:

Studies on transformation of Escherichia coli with plasmids
Published by Elsevier ,2006
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Published by Elsevier ,2006
The KpnI family of long interspersed nucleotide sequences is present on discrete sizes of circular DNA in monkey (BSC-1) cells
Journal of Molecular Biology, 1985
Some extrachromosomal circular DNAs containing the Alu family of dispersed repetitive sequences may be reverse transcripts
Journal of Molecular Biology, 1984
A new family of interspersed repetitive DNA sequences in the mouse genome
Journal of Molecular Biology, 1982
Molecular characterization of small polydisperse circular DNA from an African green monkey cell line
Biochemistry, 1982
Identification of extrachromosomal DNA in hematolymphoid cells of chickens and mice
Biochemical and Biophysical Research Communications, 1982
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
Journal of Molecular Biology, 1977
Small circular DNA in Drosophila melanogaster
Cell, 1976
Small polydisperse circular DNA of HeLa cells
Journal of Molecular Biology, 1972