EVERSION AND DIFFERENTIATION OF DROSOPHILA-MELANOGASTER LEG AND WING IMAGINAL DISKS CULTURED INVITRO WITH AN OPTIMAL CONCENTRATION OF BETA-ECDYSONE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (FEB) , 105-117
Abstract
The stages of the eversion and differentiation of prothoracic leg and wing discs of D. melanogaster, in Shields and Sang''s medium 3, are described. The range of specific imaginal structures produced, including patterns of sensilla trichodea and sensilla campaniformia, are noted, and the relationship of these structures to those differentiated in situ is discussed. Imaginal structures differentiated in vitro are smaller than those formed in situ, and in some cases patterns are incomplete. The overall level of development was satisfactory for the study of imaginal differentiation in vitro.