MONOCLONAL-ANTIBODIES THAT DETECT DIFFERENTIATION SURFACE-ANTIGENS ON HUMAN MYELOMONOCYTIC CELLS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 59  (2) , 382-392
Abstract
The production and characterization of several new [mouse] monoclonal antibodies that recognize differentiation antigens present on human cells of the myelomonocytic lineage is described. The lineage and the stage specificities of these reagents (myeloid-, monocytic-, and myelomonocytic-specific) were determined on the basis of their reactivity with human cell lines and with human peripheral blood and bone marrow cells. Cross-competition experiments demonstrated that some of the antibodies react with the same or closely associated antigenic determinants. The following 5 antigens were identified in this way: 1 present on myeloid, 1 on monocytic, and 3 on both myeloid and monocytic cells. The possible relationship of these antibodies with other established monoclonal antibodies is discussed, in addition to their use in the in vitro study of the differentiation pathways of human hemopoietic cells and in the characterization of leukemias.