Extent and Role of Urogastrone in the Adaptive Response of Rat Intestine to Patching of a Surgical Defect in the Ileum

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Abstract
Crypt cell production rate, morphometric changes and uptake of radiolabeled human urogastrone (125I rhUG) were evaluated in intestinal tissues of the rat at 2, 4, 8, and 12 days following patching of a surgical defect in the ileum with adjacent cecum. The terminal ileum was incised along its antimesenteric border and the margin of the opened ileum was sutured onto the serosal surface of the cecum. At autopsy, the animals were injected intraperitoneally with 1 microgram/g body weight of the metaphase arrest agent, vincristine sulfate, and 50 microCI of 125I-rhUG (specific activity 1.7 microCi/micrograms) to quantify the crypt cell production rate and uptake of radiolabeled urogastrone, respectively. The results indicated that the crypt cell production rate was increased significantly throughout the small intestine of operated animals till the 12th postoperative day as compared to unoperated rats. The colon showed significantly increased crypt cell production rate only on the 4th day after surgery compared to unoperated rats. The stomach showed no changes. The uptake of 125I-rhUG increased significantly on the 2nd and 4th postoperative days in the small intestine and on the 2nd postoperative day in the colon as compared to unoperated rats and gradually decreased with increasing time after surgery. These results suggest that patching of an ileal defect resulted in a strong compensatory response in the small intestine. These results also demonstrated a close association between epithelial cell proliferation and uptake of 125I-rhUG, particularly in the early part of intestinal adaptation following the surgical stimulus to ileum.(ABSTRACT TRUNCATED AT 250 WORDS)

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