Formation of D-amino-acid oxidase in the yeast Trigonopsis variabilis
- 1 July 1985
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 31 (7) , 625-628
- https://doi.org/10.1139/m85-118
Abstract
Production of D-amino-acid oxidase by T. variabilis was investigated using a 2-stage cultivation technique. After transfer of exponentially growing cells to fresh medium, the enzyme was induced by addition of D-amino acids to the growth medium, among which D-methionine and D-alanine were the most effective. The simultaneous presence of the L form of amino acids or NH4+ did not affect this induction. The presence of trace metals, Pi and a high rate of agitation were necessary for formation of maximal D-amino-acid oxidase activity. The enzyme is not subject to glucose repression.Keywords
This publication has 7 references indexed in Scilit:
- D-amino acid oxidase fromTrigonopsis variabilis: Comparison of enzyme specificity ?in vivo? and ?in vitro?Biotechnology Letters, 1985
- Regulation of L-amino acid oxidase and of D-amino acid oxidase in Neurospora crassaMolecular Genetics and Genomics, 1982
- Immobilized d-amino acid oxidaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Purification of d-amino acid oxidase from Trigonopsis variabilisAnalytical Biochemistry, 1976
- Oxidation of d -Amino Acids by a Particulate Enzyme from Pseudomonas aeruginosaJournal of Bacteriology, 1968
- Transaminase and D-Amino Acid Oxidase of Trigonopsis variabilisJournal of General Microbiology, 1962