Fibrin Membrane Endowed with Biological Function

Abstract
Transglutaminase from guinea pig liver catalyzed the formation of cross-links between fibrinogen (or fibrin) and ribonucléase. Using transglutaminase, immobilized ribonucléase was prepared by two separate methods: (1) fibrinogen-ribonuclease conjugates formed by transglutaminase were treated with thrombin to make fibrin membrane bound covalently to the enzyme; (2) fibrin polymer formed from fibrinogen with thrombin was covalently bound to ribonucléase by transglutaminase to make fibrin-ribonuclease conjugates.

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