Differences in the Physical Properties of Dense and Standard Poliovirus Particles

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Abstract
Dense poliovirus particles (DP) differ in buoyant density, sedimentation coefficient and lability from standard poliovirus particles. Dense particles band at a density of 1.44 g/ml in isopycnic CsCl gradients and sediment in sucrose gradients at 220S. When DP are centrifuged in sucrose gradients containing 1.5 M KCl, NaCl or LiCl, 2 types of particles are observed, 1 sedimenting at 220S and the other at 160S. Particles sedimenting at 220S are converted into particles sedimenting at 160S by incubation at 37.degree. C in 1.5 M KCl. The high buoyant density seems to be correlated with the high lability of DP. Dense particles are extremely labile in isotonic phosphate-buffered saline. Their degradation proceeds through an RNA-containing particle lacking polypeptide VP4 to RNA and empty capsids.

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