A systematic histological study of the features of wound healing in amputated mouse digits (191 cases) showed: (1) Provisional closure of the wound by a blood clot followed by inflammatory reactions was similar to non-amputational wounds, but differences were revealed in the patterns of tissue healing. (2) The epidermal closure of the wound preceded dermal repair, thus indicating that amputational wounds heal by primary intention. (3) The callus formations were found to differ according to the level of amputation (diaphyseal or epiphyseal) of the phalange. (4) Morphologically well-defined, but only transitory bursa-like formations, were observed to form between the cut bone and the amputated surface. (5) Within 2 weeks after amputation, the skin was reformed; complete with dermal and subdermal tissue and hair follicles the return of the amputation stump to morphological equilibrium was invariably accompanied by the emergence of a heavy multiple-layered pad of connective tissue capping the distal and lateral parts of the cut bone. (6) A comparison of the histological features during wound healing in amputated mouse digits with those in amputated, non-regenerating limbs of Anura reveals striking similarities.