A High-Throughput Assay for siRNA-Based Circadian Screens in Human U2OS Cells
Open Access
- 20 October 2008
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 3 (10) , e3457
- https://doi.org/10.1371/journal.pone.0003457
Abstract
The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.Keywords
This publication has 30 references indexed in Scilit:
- Tuning gene expression to changing environments: from rapid responses to evolutionary adaptationNature Reviews Genetics, 2008
- Disruption of the circadian clock due to theClockmutation has discrete effects on aging and carcinogenesisCell Cycle, 2008
- The Clock Proteins, Aging, and TumorigenesisCold Spring Harbor Symposia on Quantitative Biology, 2007
- Early aging and age-related pathologies in mice deficient in BMAL1, the core componentof the circadian clockGenes & Development, 2006
- When the Clock stops ticking, metabolic syndrome explodesNature Medicine, 2006
- A Rhythmic RorNeuron, 2004
- A clockwork web: circadian timing in brain and periphery, in health and diseaseNature Reviews Neuroscience, 2003
- Circadian rhythms from flies to humanNature, 2002
- Coordinated Transcription of Key Pathways in the Mouse by the Circadian ClockCell, 2002
- Molecular Bases of Circadian RhythmsAnnual Review of Cell and Developmental Biology, 2001