Molecular characterization of dioxygenases from polycyclic aromatic hydrocarbon-degrading Mycobacterium spp.
- 1 June 2003
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 223 (2) , 177-183
- https://doi.org/10.1016/s0378-1097(03)00328-8
Abstract
Polycyclic aromatic hydrocarbon (PAH)-degrading genes nidA and nidB that encode the alpha and beta subunits of the aromatic ring-hydroxylating dioxygenase have been cloned and sequenced from Mycobacterium vanbaalenii PYR-1 [Khan et al., Appl. Environ Microbiol. 67 (2001) 3577-3585]. In this study, the presence of nidA and nidB in 12 other Mycobacterium or Rhodococcus strains was investigated. Initially, all strains were screened for their ability to degrade PAHs by a spray plate method, and for the presence of the dioxygenase Rieske center region by polymerase chain reaction (PCR). Only Mycobacterium sp. PAH 2.135 (RJGII-135), M. flavescens PYR-GCK (ATCC 700033), M. gilvum BB1 (DSM 9487) and M. frederiksbergense FAn9T (DSM 44346), all previously known PAH degraders, were positive in both tests. From the three positive strains, complete open reading frames of the nidA and nidB genes were amplified by PCR, using primers designed according to the known nidA and nidB sequences from PYR-1, cloned in the pBAD/Thio-TOPO vector and sequenced. The sequences showed >98% identity with the M. vanbaalenii PYR-1 nidA and nidB genes. Southern DNA-DNA hybridization using nidA and nidB probes from PYR-1 revealed that there is more than one copy of nidA and nidB genes in the strains PYR-1, BB1, PYR-GCK and FAn9T. However, only one copy of each gene was observed in PAH2.135.Keywords
This publication has 59 references indexed in Scilit:
- Molecular Cloning, Nucleotide Sequence, and Expression of Genes Encoding a Polycyclic Aromatic Ring Dioxygenase from Mycobacterium sp. Strain PYR-1Applied and Environmental Microbiology, 2001
- Isolation and Characterization of the Genes Encoding a Novel Oxygenase Component of Angular Dioxygenase from the Gram-Positive Dibenzofuran-Degrader Terrabacter sp. Strain DBF63Biochemical and Biophysical Research Communications, 2001
- Cloning, Expression, and Characterization of the katG Gene, Encoding Catalase-Peroxidase, from the Polycyclic Aromatic Hydrocarbon-Degrading Bacterium Mycobacterium sp. Strain PYR-1Applied and Environmental Microbiology, 2000
- A Novel Phenanthrene Dioxygenase from Nocardioides sp. Strain KP7: Expression in Escherichia coliJournal of Bacteriology, 2000
- Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24Applied Microbiology and Biotechnology, 1999
- Involvement of the Terminal Oxygenase β Subunit in the Biphenyl Dioxygenase Reactivity Pattern toward ChlorobiphenylsJournal of Bacteriology, 1998
- Structure of an aromatic-ring-hydroxylating dioxygenase – naphthalene 1,2-dioxygenaseStructure, 1998
- Environmental Quality Objectives for 10 Polycyclic Aromatic Hydrocarbons (PAHs)Ecotoxicology and Environmental Safety, 1997
- Atsdr Evaluation of Health Effects of Chemicals. Iv. Polycyclic Aromatic Hydrocarbons (PAHs): Understanding a Complex ProblemToxicology and Industrial Health, 1996
- THE ELECTRON-TRANSPORT PROTEINS OF HYDROXYLATING BACTERIAL DIOXYGENASESAnnual Review of Microbiology, 1992