VITAL STAINING OF THE CENTRIFUGED ARBACIA PUNCTULATA EGG

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Abstract
The different layers of the Arbacia egg stratified by centrifugal force are stained differentially by different vital dyes. The jelly surrounding the egg is stained with Janus green (purple), Janus dark blue B (purple) and toluidin blue (pinkish lavender). The oil and nucleus are not stained. The clear layer is slightly stained with Bismarck brown (yellow), chrysoidin (yellow), Nile blue sulphate (blue), toluidin blue (pinkish lavender), rhodamine (pink) and neutral red (pinkish yellow). With certain dyes, the stain is more intense in the upper or lower portion of the clear layer. The mito-chondrial layer is stained differentially with methyl green (purple), gentian violet (purple) and Janus green (blue). Yolk and pigment are stained with methylene blue (blue), brilliant cresyl blue (blue), Nile blue (blue), toluidin blue (purple), methyl violet (purple), rhodamine (red), neutral red (red), Bismarck brown (yellow brown) and chrysoidin (yellow brown). With saffranin O, the pigment is stained blood red, the yolk is unstained.