An improved assay method for neutralizing antibodies against influenza viruses

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Abstract
1. A method has been developed for the assay of antisera against influenza viruses. 2. Surviving bits of allantois-on-shell serve as host tissue, eighty to one hundred units being provided by a single egg. The test is set up in plastic trays, and uses a simple synthetic medium. 3. The method has worked equally well with the ten representative strains of influenza virus tested. It surpasses the orthodox techniques of neutralization in mice or whole eggs as regards sensitivity, accuracy and economy.

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