The isolation of human plasma prekallikrein

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Abstract
1 . The isolation of human plasma prekallikrein was achieved by fractionating human plasma on diethylaminoethyl cellulose (DEAE) in the presence of heparin. 2 . Heparin was shown to inhibit the activation of prekallikrein during the isolation procedure. 3 . The isolated prekallikrein fraction had some kallikrein activity which could be inhibited by diisopropylfluorophosphate (DFP) without affecting the ability of prekallikrein to be activated. 4 . The prekallikrein obtained was functionally pure in that it had no kallikrein inhibiting or activating activity. It was not physico-chemically pure, the major contaminant being the immunoglobulin IgG.