Superoxide dismutase in vesicular arbuscular‐mycorrhizal red clover plants

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Abstract
The isoenzymatic pattern of Superoxide dismutase (SOD; EC 1.15.1.1) was studied in the symbiosis of Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe‐Trifolium prarense L A Cu.Zn‐SOD (M, 40500) was found in spores of G. mosseae. while one Mn‐SOD (Mn‐SOD I) and two Cu.Zn‐SODs (Cu.Zn‐SOD 1 and Cu.Zn‐SOD II) were present in both roots and leaves of T. pratense. Molecular masses for Cu.Zn‐SOD I and Cu.Zn‐SOD II were 31000 and 34300. respectively. However, when T. prateme and G. mosseae were associated, mycorrhizal roots showed two new iso‐zymes, Mn‐SOD II and mycCu.Zn‐SOD, which have relative molecular masses of 37 800 and 33 300, respectively. The mycCu.Zn‐SOD was found to be specific for this association, whereas Mn‐SOD II was also present in nodules of Rhizobium‐T. pra‐tense. Results suggest that both enzymes are induced in the T. praiense roots in response to invasion by mycorrhizal fungi, perhaps as a result of an increase in the generation of O‐2 radicals in plant roots.