Abstract
Calcium-magnesium-free perfusions induced a large release of lactate dehydrogenase from isolated rat heart after returning to normal perfusion. This release generally appeared without irreversible damage and was quantitatively dependent on the duration and the temperature of the abnormal perfusion. Mechanical activity and the lack thereof are important factors in determining enzyme release. It is suggested that lactate dehydrogenase release does not result from a paradoxical effect of calcium but is dependent on the recovery of contraction in a damaged tissue.

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