Changes in Lipid Values and Lipoprotein Patterns of Scrum Samples Contaminated with Bacteria

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Abstract
Phospholipase C-producing bacilli were found as contaminants in 3% of serum samples received by mail for lipid analysis. The contamination results in apparent increases in serum triglyceride values and chylomicrons, with corresponding decreases in serum phospholipid values and alpha, beta, and prebeta lipoproteins. The changes in contaminated sera were confirmed with phospholipase C isolated from Clostridium perfringens. The results suggest that the phosphorus portion of the phospholipid molecule is exposed to the aqueous environment and is essential for the stabilization characteristics and electrophoretic properties of lipoproteins. Ethylenediamine tetraacetic acid, which inhibits phospholipase C, was found to be effective in preserving the structural integrity of serum lipids and lipoproteins.

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