FUNCTIONAL CHARACTERIZATION OF AN LCCL–LECTIN DOMAIN CONTAINING PROTEIN FAMILY IN PLASMODIUM BERGHEI

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Abstract
Using bioinformatic, proteomic, immunofluorescence, and genetic cross methods, we have functionally characterized a family of putative parasite ligands as potential mediators of cell–cell interactions. We name these proteins the Limulus clotting factor C, Coch-5b2, and Lgl1 (LCCL)–lectin adhesive–like protein (LAP) family. We demonstrate that this family is conserved amongst Plasmodium spp. It possesses a unique arrangement of adhesive protein domains normally associated with extracellular proteins. The proteins are expressed predominantly, though not exclusively, in the mosquito stages of the life cycle. We test the hypothesis that these proteins are surface proteins with 1 member of this gene family, lap1, and provide evidence that it is expressed on the surface of Plasmodium berghei sporozoites. Finally, through genetic crosses of wild-type Pblap1+ and transgenic Pblap1− parasites, we show that the null phenotype previously reported for sporozoite development in a Pblap1− mutant can be rescued within a...