A mitochondrial locus is necessary for the synthesis of mitochondrial tRNA in the yeast Saccharomyces cerevisiae.
- 1 August 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (8) , 4743-4747
- https://doi.org/10.1073/pnas.78.8.4743
Abstract
A cloned yeast mitochondrial .**GRAPHIC**. gene was used as a probe to detect RNA species that are transcripts from this gene in wild-type S. cerevisiae and in petite deletion mutants. In RNA from wild-type cells, the tRNA is the most prominent transcript of the gene. In RNA from deletion mutants that retain this gene but have lost other regions of mtDNA [mitochondrial DNA], high MW transcripts containing the .**GRAPHIC**. sequences accumulate but .**GRAPHIC**. is not made. .**GRAPHIC**. synthesis can be restored in these mutants when they are mated to other deletion mutants that retain a different portion of the mitochondrial genome. Protein synthesis is not necessary for the restoration, and the restoration is not due to a nuclear effect or to an effect of mating alone, because strains without mtDNA are not able to restore tRNA synthesis. A yeast mitochondrial locus that is necessary for tRNA synthesis exists. Because the restoration of .**GRAPHIC**. synthesis appears to result from intergenic complementation, not recombination, this locus probably acts in trans.This publication has 34 references indexed in Scilit:
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