Atg21p is essential for macropexophagy and microautophagy in the yeastHansenula polymorpha
- 2 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 577 (3) , 491-495
- https://doi.org/10.1016/j.febslet.2004.10.055
Abstract
ATG genes are required for autophagy‐related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp‐Atg21p, fused to eGFP, had a dual location in the cytosol and in peri‐vacuolar dots. We demonstrate that Hp‐Atg21p is essential for two separate modes of peroxisome degradation, namely glucose‐induced macropexophagy and nitrogen limitation‐induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.Keywords
This publication has 25 references indexed in Scilit:
- Atg21 Is Required for Effective Recruitment of Atg8 to the Preautophagosomal Structure during the Cvt PathwayJournal of Biological Chemistry, 2004
- Atg21 Is a Phosphoinositide Binding Protein Required for Efficient Lipidation and Localization of Atg8 during Uptake of Aminopeptidase I by Selective AutophagyMolecular Biology of the Cell, 2004
- The (strain CBS4732) genome sequencing and analysisFEMS Yeast Research, 2003
- Transcriptional Down-regulation of Peroxisome Numbers Affects Selective Peroxisome Degradation in Hansenula polymorphaJournal of Biological Chemistry, 2003
- Tagging Hansenula polymorpha genes by random integration of linear DNA fragments (RALF)Molecular Genetics and Genomics, 2001
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals.The Journal of cell biology, 1994
- Highly-efficient electrotransformation of the yeast Hansenula polymorphaCurrent Genetics, 1994
- Chromosomal targeting of replicating plasmids in the yeast Hansenula polymorphaJournal of General Microbiology, 1992
- Growth of Hansenula polymorpha in a methanol-limited chemostatArchiv für Mikrobiologie, 1976