Recruitment of enzyme activity in albumin by molecular imprinting
- 1 December 1996
- journal article
- research article
- Published by Wiley in Macromolecular Rapid Communications
- Vol. 17 (12) , 871-874
- https://doi.org/10.1002/marc.1996.030171205
Abstract
The catalytic activity of bovine serum albumin (BSA) modified physically by molecular imprinting using transition‐state analogue (TSA) as a template molecule was studied. The resultant imprinted serum albumin (Imp‐BSA) showed the rate acceleration of dehydrofluorination reaction from (4R,4S)‐4‐fluoro‐4‐(4‐nitrophenyl)butan‐2‐one (1) and followed the type of Michaelis‐Menten reaction in ethyl acetate solution. The enzymatic activity of Imp‐BSA was competitively inhibited by (4R,4S)‐4‐hydroxy‐4‐(4‐nitrophenyl)butan‐2‐one (4).Keywords
This publication has 11 references indexed in Scilit:
- Molecular Imprinting in Cross‐Linked Materials with the Aid of Molecular Templates— A Way towards Artificial AntibodiesAngewandte Chemie International Edition in English, 1995
- Molekulares Prägen (Imprinting) in vernetzten Materialien mit Hilfe von Matrizenmolekülen – auf dem Weg zu künstlichen AntikörpernAngewandte Chemie, 1995
- A New Method for the Introduction of Recognition Site Functionality into Polymers Prepared by Molecular Imprinting: Synthesis and Characterization of Polymeric Receptors for CholesterolJournal of the American Chemical Society, 1995
- Mechanistic Studies of an Antibody-Catalyzed Elimination ReactionJournal of the American Chemical Society, 1994
- Molecular imprinting of proteins and other macromolecules resulting in new adsorbentsBiotechnology & Bioengineering, 1992
- At the Crossroads of Chemistry and Immunology: Catalytic AntibodiesScience, 1991
- A new strategy for the generation of catalytic antibodiesNature, 1989
- Antibody catalysis approaching the activity of enzymesJournal of the American Chemical Society, 1988
- Selective Chemical Catalysis by an AntibodyScience, 1986
- Catalytic AntibodiesScience, 1986