INVITRO TRANSFER RATE OF C-14 FROM ACETATE-1-C-14 INTO OVARIAN STEROIDS IN RAT OVARY DURING ESTROUS-CYCLE AND EFFECTS OF LH AND FSH

  • 1 January 1976
    • journal article
    • research article
    • Vol. 67  (2) , 172-183
Abstract
Fluctuations of ovarian biosynthetic activity and effects of exogenous LH [luteinizing hormone] and FSH [follicle-stimulating hormone] on it during the estrous cycle were investigated by measuring in vitro transfer rates of 14C from 14C-l-acetate into progesterone (P), 20.alpha.-hydroxy-pregn-4-en-3-one (20.alpha.-OH-P) and estrogen (estradiol and estrone, E) in the ovarian homogenates from rats autopsied at 2 h intervals. The transfer rate of 14C from 14C-l-acetate into P was lowest in the afternoon of estrus and increased from the morning of diestrus 1, making its peaks during the afternoon of diestrus 2 and in the midnight of proestrus. The transfer of 14C into 20.alpha.-OH-P was high on the days of diestrus 2 and proestrus, with its peak in the afternoon of the latter day. The maximum transfer of 14C into E in the afternoon of proestrus and a high rate in the morning of estrus with a relatively low one in the midnight were observed. Exogenously injected LH (150 .mu.g) or FSH (150 .mu.g) was either stimulatory or inhibitory to the transfer rates of 14C from 14C-l-acetate into ovarian steroids. During day time of diestrus 2 and midnight between proestrus and estrus, the transfer of 14C into P and 20.alpha.-OH-P was increased by LH, and during day time of proestrus and from the afternoon of estrus to the morning of diestrus 1 was decreased. The transfer of 14C into E was increased by LH from the afternoon of diestrus 2 to the morning of proestrus and was decreased during the afternoon of proestrus and from the afternoon of estrus to the morning of diestrus 2. Administration of FSH was stimulatory or inhibitory. The 14C transfer into P and 20.alpha.-OH-P was increased by FSH from the afternoon of estrus to the morning of proestrus, but in the afternoon of proestrus they decreased. Transfer of 14C into E was increased by FSH significantly on the days of diestrus 2 and proestrus and slightly on the day of estrus, while it decreased in the afternoon of diestrus 1.

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