Array painting using microdissected chromosomes to map chromosomal breakpoints
- 1 February 2007
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 116 (3) , 158-166
- https://doi.org/10.1159/000098181
Abstract
Molecular characterization of breakpoints of chromosomal rearrangements is a successful strategy for the identification of candidate disease genes. Mapping translocation breakpoints and rearranged chromosomal boundaries is labor intensive and/or time consuming. Here, we present a novel and rapid procedure to map such chromosomal breakpoints by hybridizing amplified microdissection derived DNA of aberrant chromosomes to arrays containing genomic clones. We illustrate the potential of the technique by molecularly delineating the breakpoints in five small supernumerary marker chromosomes (sSMC) and mapping the breakpoints of five different chromosomal translocations.Keywords
This publication has 28 references indexed in Scilit:
- The complex nature of constitutional de novo apparently balanced translocations in patients presenting with abnormal phenotypesJournal of Medical Genetics, 2005
- Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primerPublished by Elsevier ,2004
- Small supernumerary marker chromosomes (sSMC) in humansCytogenetic and Genome Research, 2004
- Paint-assisted microdissection-FISH: Rapid and simple mapping of translocation breakpoints in the embryonal rhabdomyosarcoma cell line RDCytometry, 2004
- Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarraysJournal of Medical Genetics, 2003
- Identification of a 650 kb duplication at the X chromosome breakpoint in a patient with 46,X,t(X;8)(q28;q12) and non-syndromic mental retardationJournal of Medical Genetics, 2003
- Methodologies in cancer cytogenetics and molecular cytogeneticsAmerican Journal of Medical Genetics, 2002
- A cryptic deletion of 2q35 including part of the PAX3 gene detected by breakpoint mapping in a child with autism and a de novo 2;8 translocationJournal of Medical Genetics, 2002
- GATA3 haplo-insufficiency causes human HDR syndromeNature, 2000
- Rapid generation of region-specific genomic clones by chromosome microdissection: Isolation of DNA from a region frequently deleted in malignant melanomaGenomics, 1992