DNA-cellulose column chromatography of phosphorylated nucleolar nonhistone proteins
- 1 January 1976
- journal article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 10 (1) , 3-9
- https://doi.org/10.1007/bf01731675
Abstract
A salt-extraction procedure was used to isolate a nucleolar nonhistone protein fraction, containing [32P]phosphoserine, from the nucleoli of Novikoff hepatoma ascites cells. These proteins are similar in amino-acid composition to whole nuclear (chromosomal) nonhistone proteins. DNA-cellulose column chromatography showed that this fraction contains DNA-binding phosphoproteins, some of which will bind only to homologous (Novikoff) nucleolar or nuclear DNA.Keywords
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