Improvements to the Chemical Synthesis of Biologically Active RNA Using 2'-O-Fpmp Chemistry

1 May 1995

journal article
Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids

Vol. 14 (3) , 911-915
https://doi.org/10.1080/15257779508012500

Abstract

The synthetic cycle protocol for the solid phase synthesis of RNA using 5′-O-(DMTr)-2′-O-(Fpmp)-ribonucleoside phosphoramidites is optimised. A simple and reliable two step deprotection procedure is developed to isolate biologically active RNA. It is demonstrated that fully deprotected RNA is completely stable under the deprotection conditions and that it does not undergo internucleotide cleavage and/or migration. Ribozymes and substrate RNAs synthesized using this chemistry were found to be catalytically active.

Keywords

BIOLOGICALLY ACTIVE
CHEMICAL SYNTHESIS
FPMP CHEMISTRY
ACTIVE RNA
RNA USING
IMPROVEMENTS

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