The effect of ultrasound and its combination with radiation on the genetic material ofSaccharomyces cerevisiae
- 1 May 1978
- journal article
- research article
- Published by Springer Nature in Folia Microbiologica
- Vol. 23 (3) , 216-224
- https://doi.org/10.1007/bf02876582
Abstract
Ultrasonication at 20 kHz, intensity 35 W/cm2 and amplitude 15–25 μm of a diploid strain ofSaccharomyces cerevisiae was found to act as a weak mutagen with maximum efficiency at the 20 % survival of the cells. Under these conditions, the frequency of reversion of the suppressible allele ilv1-92 increased ten times, the frequency of mitotic gene conversion four times. Doses leading to survivals lower than 20 % led to a slight increase in the frequency of cytoplasmic respiration-deficient mutants. Submutagenic doses applied immediately after γradiation or UV light did not substantially increase the effect of these physical agents on the genetic material of the yeast strain investigated. Application of ultrasound prior to UV radiation did not considerably influence the effect of the radiation either.Keywords
This publication has 15 references indexed in Scilit:
- A yeast strain for simultaneous detection of induced mitotic crossing over, mitotic gene conversion and reverse mutationMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
- An assessment of ultrasonic radiation hazard using yeast genetic systemsThe British Journal of Radiology, 1974
- A yeast strain for visual screening for the two reciprocal products of mitotic crossing overMutation Research/Environmental Mutagenesis and Related Subjects, 1973
- Suppressor studies on ilv1 mutants of Saccharomyces cerevisiaeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- An approach to the mechanism of killing of cells in suspension by ultrasoundBiochimica et Biophysica Acta (BBA) - General Subjects, 1973
- Transformation with sonicated DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Mapping ultrasonic fields with cholesteric liquid crystalsUltrasonics, 1971
- Chemical mechanism of sonic, acid, alkaline and enzymic degradation of DNAJournal of Molecular Biology, 1965
- Études sur la suppressivité des mutants a deficience respiratoire de la levureHeredity, 1965
- Studies on the Sonic Degradation of Deoxyribonucleic AcidBiophysical Journal, 1962