The detection of PR (b) protein and TMV by ELISA in systemic and localised virus infections of tobacco
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 4 (1) , 55-60
- https://doi.org/10.1007/bf02498715
Abstract
An antiserum was prepared to the b1 protein purified from TMV infectedN. tabacum cv. Xanthi-nc leaves and used to study PR proteins. The Xanthi-nc proteins b2 and b3 were shown to be serologically closely related to b1. Antisera to b1 protein and TMV were used in a F(ab′)2 enzyme linked immunosorbent assay to monitor PR protein and TMV concentrations, respectively, during the first 6 days of a systemic TMV infection (cv. Xanthi) and a localised TMV infection (cv. Xanthi-nc).Keywords
This publication has 12 references indexed in Scilit:
- Biochemical and serological characterization of b-proteins fromNicotiana speciesPlant Molecular Biology, 1985
- A Simple Indirect ELISA using F(ab')2 Fragments of ImmunoglobulinJournal of General Virology, 1982
- Regulation of Changes in Proteins and Enzymes Associated with Active Defence against Virus InfectionPublished by Springer Nature ,1982
- Evidence for the occurrence of the “pathogenesis-related” proteins in leaves of healthy tobacco plants during floweringPhysiological Plant Pathology, 1981
- Lesion growth and virus localization in leaves of Nicotiana tabacum cv. Xanthi nc. after inoculation with tobacco mosaic virus and incubation alternately at 22°C and 32°CPhysiological Plant Pathology, 1981
- 3,3′,5,5′-Tetramethylbenzidine as an Ames Test Negative Chromogen for Horse-Radish Peroxidase in Enzyme-ImmunoassayJournal of Immunoassay, 1981
- Antigen controlled immuno diagnosis—‘Acid test’Journal of Immunological Methods, 1980
- The Effects of Aspirin and Polyacrylic Acid on Soluble Leaf Proteins and Resistance to Virus Infection in Five Cultivars of TobaccoJournal of Phytopathology, 1980
- Comparison of Three Pathogenesis-related Proteins from Plants of Two Cultivars of Tobacco Infected with TMVJournal of General Virology, 1980
- Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold SuspensionsNature Physical Science, 1973