Permeabilization of Cinchona ledgeriana cells by dimethylsulphoxide. effects on alkaloid release and long-term membrane integrity
- 1 December 1984
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 3 (6) , 262-265
- https://doi.org/10.1007/bf00269308
Abstract
Dimethyl sulphoxide (DMSO) has been used to permeabilize cells of Cinchona ledgeriana in suspension culture and promote the release of intracellular alkaloids. 5–6% v/v is required before any release is seen, and greater than 20% DMSO is required for full release. Even at these high levels of DMSO release is slow, taking in excess of seven hours to reach completion. Conditions which produce significant release of alkaloids have a deleterious effect on cells. Many of the membranes permeabilized did not recover their ability to selectively exclude compounds such as mannitol when the DMSO was removed. It is concluded that DMSO is not a suitable material for inducing alkaloid release in any biotechnological exploitation of alkaloid production by C. ledgeriana.Keywords
This publication has 17 references indexed in Scilit:
- A novel method for the immobilisation and culture of plant cellsFEBS Letters, 1983
- Permeabilization of immobilized plant cells, resulting in release of intracellularly stored products with preserved cell viabilityApplied Microbiology and Biotechnology, 1983
- Exploitation of plant cells for the production of natural compoundsBiotechnology & Bioengineering, 1982
- Permeabilized cellsAnalytical Biochemistry, 1982
- Enzyme activities of the primary and secondary metabolism of simultaneously permeabilized and immobilized plant cellsAnalytical Biochemistry, 1981
- The Rapid Isolation of Vacuoles from Leaves of Crassulacean Acid Metabolism PlantsPlant Physiology, 1980
- Dimethylsulfoxide as a Potential Tool for Analysis of Compartmentation in Living Plant CellsPlant Physiology, 1979
- An Investigation of Factors which Influence the Production of Abnormal Terpenoids by Callus Cultures ofAndrographis paniculataNeesJournal of Experimental Botany, 1971
- The Effects of Amino Acids and Ammonium on the Growth of Plant Cells in Suspension CulturePlant Physiology, 1970
- Vacuoles of Chelidonium Latex: Lysosomal Property and Accumulation of AlcaloidsBiochemie und Physiologie der Pflanzen, 1970