The Hydrolysis of Tween 80 by Vibrios and Aeromonads

Abstract
Summary.: A total of 307 strains consisting of 89Vibrio cholerae, 37 NAG vibrios, 4Aeromonassp., 20Pseudomonas aeruginosa, 47 shigellae, 59Escherichia coli, 27Proteus mirabilisand 25 salmonellae were examined for their opacity reaction in Tween 80 agar medium. Strong reactions occurred with 87V. cholerae, 26 NAG vibrios, 2Aeromonasand 5Pseudomonasstrains; and weak and slow positive reactions were noted in only 6 strains of NAG vibrios and 3 ofPseudomonas; no reaction was observed among the remaining strains. The study showed that Tween 80 hydrolysis can be used as a test for characterization of theVibrioandAeromonasgroups and for their differentiation from the Enterobacteriaceae.

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