Method for Staining Bacterial Flagella
- 1 January 1931
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 6 (2) , 43-45
- https://doi.org/10.3109/10520293109116041
Abstract
The following method is recommended for use on smears made from suspensions of 10-16-hr. agar-slant cultures, incubated 30 min. at 37[degree] C. before spreading on cleaned and flamed slides: (1) Cover with fixative (100 cc. of 1/4 saturated aqueous solution picric acid, with 5 gm. tannic acid and 7.5 gm. ferrous sulphate). 2. Wash with tap-water, dry, and cover with Fontana spirochaete stain; heat to steaming and allow to act for 1-2 min. Wash in tap-water. The stain is prepared as follows: To 25 cc. 2% AgNOs add dilute NH3 till the precipitate which forms redissolves; then add more AgNOs till a faint turbidity results. A clear solution is useless.This publication has 0 references indexed in Scilit: