The in vitro phosphorylation of actin from rat cerebral cortex
- 1 July 1983
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 8 (7) , 865-871
- https://doi.org/10.1007/bf00964547
Abstract
Actin was phosphorylated by a cyclic AMP-stimulated protein kinase in a lysed synaptosomal fraction incubated with [γ-32P]ATP, while calcium had no effect on endogenous labeling of the protein. Incubation of an intact synaptosomal fraction with32P-inorganic phosphate did not lead to any detectable phosphorylation of actin in the presence or absence of dibutryl-cyclic AMP, or chemical depolarization. It is suggested that actin is not phosphorylated in the physiologically relevant intact synaptosomes but gains access to protein kinases on lysis.This publication has 17 references indexed in Scilit:
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