The Coexistence of Androgen and Glucocorticoid Receptors in the DDT1Cloned Cell Line1

Publisher Website
Google Scholar
Abstract
The hamster ductus deferens cloned tumor cell line (DDT1) contained both androgen and glucocorticoid binding activity. The androgen receptor binding site concentration is 1.07 .times. 10-13 mol of testosterone (T)/mg protein, and binds with a Kd of 4.3 .times. 10-10 M. Dihydrotestosterone (DHT) is also bound to the receptor with a Kd of 2.99 .times. 10-10 M, and the binding site concentration is 1.33 .times. 10-13 mol/mg protein. The order of steroid binding affinity is DHT > T > estradiol > progesterone. Cortisol, dexamethasone and triamcinolone acetonide do inhibit the androgen binding in vivo or in vitro. In a cell free system antiandrogens inhibit binding of DHT. DDT1 cells have a separate receptor for cortisol which binds at saturation 3.44 .times. 10-13 mol cortisol/mg protein and has a Kd of 4.54 .times. 10-9 M. These endocrine target cells apparently contain specific high affinity receptors for more than 1 type of steroid. The glucocorticoid receptor may be important for maintaining essential undifferentiated functions, while the DHT receptor gives the specific characteristics of sex hormone responsive tissues. [Fetal bovine serum was used].

This publication has 2 references indexed in Scilit: