Glucoamylase Production by Aureobasidium pullulans
- 1 July 1984
- Vol. 76 (4) , 643-649
- https://doi.org/10.2307/3793221
Abstract
A. pullulans converts starch into biomass by the production of glucoamylase (1,4-.alpha.-D-glucan glucohydrolase EC 3.2.1.3) only. The enzyme synthesis apparently did not depend on substrate induction; maximal enzyme activity, however, occurred after 48 h growth in YNB [yeast N base] medium containing soluble starch as the sole C source. The enzyme had the following characteristics, as determined in the cell-free culture broth: optimal pH 5.75; optimal temperature 50.degree. C; complete heart inactivation after 10 min at 60.degree. C; apparent optimal reaction time 120 min.This publication has 6 references indexed in Scilit:
- Extracellular Enzymatic Activities in Aureobasidium pullulansMycologia, 1982
- Inorganic nitrogen nutrition and yeast-mycelial dimorphism in Aureobasidium pullulansTransactions of the British Mycological Society, 1982
- Measurement of α-amylase and glucoamylase activities produced during fermentationEnzyme and Microbial Technology, 1980
- The Transition from Yeast-like to Chlamydospore Cells in Pullularia pullulansJournal of General Microbiology, 1978
- Influence of carbon and nitrogen sources on the morphology of Pullularia pullulansTransactions of the British Mycological Society, 1977
- A PHOTOMETRIC METHOD FOR THE DETERMINATION OF α-AMYLASE IN BLOOD AND URINE, WITH USE OF THE STARCH-IODINE COLORJournal of Biological Chemistry, 1949