Anticoagulant activity of dermatan polysulfates.

Publisher Website
Google Scholar
Abstract
Dermatan polysulfates (DPS) I, II, and III isolated from hagfish notochord, hagfish skin and shark skin, respectively, and chemically sulfated dermatan sulfate exhibit considerable anticoagulant activity in the activated partial thromboplastin time (APTT) system. On comparing the activities with the various compositions, including disaccharide units produced by the digestion with chondroitinase-ABC, the activity of these dermatan polysulfates evidently depends not only on the total sulfate content but also on the content of sulfated L-iduronic acid residues. The activity apparently decreased for MW of < 10,000. The effect of these dermatan polysulfates on the inactivation of the clotting enzymes, factor Xa and thrombin, by antithrombin III (AT-III) was studied using chromogenic substrates for the assay of the enzyme activities. The dermatan polysulfates showed an inhibitory effect on thrombin-AT-III, as estimated by the APTT assay, in contrast with the effect on factor Xa-AT-III which was small.