Negative Phototactic Activity Used to Determine Larval Toxicity of Insecticides to Diabrotica virgifera12

Abstract
A technique to obtain objective mortality data for larvae of the western corn rootworm treated with diazinon, Bux® [m- (l-ethylpropyl) phenyl methylcarbamate mixture with m- (l-methylbutyl) phenyl methylcarbamate], and phorate is detailed. The use of negative phototactic larval activity following insecticide treatment was more consistant than determining death by observation. For diazinon, the percentage differences in LD50 values between larvae and adults (phototactic technique) for 1974 was 1.04. Differences in LD50 values (observation) between larvae and adults for 1962 and 1969 were 51.6 and 17.0, respectively.

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