Anticoagulantly active heparin-like molecules from vascular tissue

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Abstract
Mucopolysaccharides were isolated from calf cerebral microvasculature and calf aorta. The only complex carbohydrates that exhibited anticoagulant activity were heparin-like components. The biologic potencies of calf cerebral and aortic heparin-like species were 2.92 U/mg of antifactor Xa activity and 2.85 U/mg of anti-factor IIa activity, as well as 0.56 U/mg of anti-factor Xa activity and 0.19 U/mg of anti-factor IIa activity, respectively. The anticoagulantly active aortic components were significantly present only within the intima. The above populations of heparin-like species were affinity fractionated with antithrombin. The highly active component obtained from calf cerebral microvascular exhibited an anti-factor Xa activity of 40.7 U/mg as well as an antifactor IIa activity of 36.8 U/mg, constituted .apprx. 4.2% of the initial mass of the starting material, and represented .apprx. 75% of the biologic potency of the starting material. The highly active component derived from calf aorta exhibited an anti-factor Xa activity of 55.4 U/mg as well as an antifactor IIa activity of 11.3 U/mg, constituted .apprx. 0.3% of the initial mass of the starting material, and represented .apprx. 60% of the biologic potency of the starting material. The highly active cerebral microvascular species possessed a MW and charge density similar to that of heparan sulfate whereas the highly active aortic species displayed a MW and charge density equivalent to that of a hexadecasaccharide fragment of heparin.

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