Die Chemie der Tuberkelbazillen-Lipoide. XL. Über das Vorkommen von Sterinen in Tuberkelbazillen.
- 1 January 1935
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 237 (1-3) , 40-45
- https://doi.org/10.1515/bchm2.1935.237.1-3.40
Abstract
450-600 gm. lots of 5 strains of human tubercle bacilli grown on Long''s synthetic medium were exhaustively extracted with lipoid solvents; the defatted bacteria dissolved in boiling 10% KOH and the solution extracted with ether. Unsaponifiable portions of all lipoid fractions were examined for sterols by digitonin precipitation and the Liebermann-Burchard reaction. Only acetone-soluble fat and wax yielded traces of sterols, 1 part in 624,000 parts dry bacteria from the fat and 1 in 870,000 from the wax; such amts. are probably due to accidental contamination. The tubercle bacillus does not produce sterols.This publication has 4 references indexed in Scilit:
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- Zur Frage des Steringehaltes von Bakterien. (Nachtrag: Untersuchungen an Colibazillen.)Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1935
- THE CHEMISTRY OF THE LIPOIDS OF TUBERCLE BACILLIJournal of Biological Chemistry, 1929
- THE SEPARATION OF LIPOID FRACTIONS FROM TUBERCLE BACILLIJournal of Biological Chemistry, 1927