Simultaneous Measurement of Membrane Capacitance and Whole Cell Currents during Cell Swelling in Macrovascular Endothelium
- 31 December 1996
- journal article
- research article
- Published by S. Karger AG in Cellular Physiology and Biochemistry
- Vol. 7 (1) , 19-24
- https://doi.org/10.1159/000154848
Abstract
We have used the whole-cell patch-clamp technique, combined with membrane capacitance (Cm) measurements to study the activation mechanism of swelling-induced Cl– currents in cultured endothelial cells from bovine pulmonary artery (CPAE cells). Changes in CM during cell swelling were less than 2% of its initial value in isotonic solution in 77% of the cells (1.1 ± 0.2%, n =17). In the remaining 23% of the cells, changes in Cm ranged between 4 and 13% (10.6 ± 0.0%, n = 5). Cell swelling activated in all cells an outwardly rectifying Cl– current, which was correlated with the changes in cell volume but not with the changes in Cm.The Cl– current was also not affected if vesicular transport was inhibited with brefeldin A. Current activation and deactivation preceded the changes in Cm in those cells where such changes have been observed. It is concluded that activation of the swelling-induced Cl– current in endothelial cells is not due to incorporation of ion channels by vesicle fusion.Keywords
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