Purification and Characterization of Genetically Polymorphic Deoxyribonuclease I from Human Kidney1
- 1 September 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 110 (3) , 321-323
- https://doi.org/10.1093/oxfordjournals.jbchem.a123578
Abstract
Deoxyribonuclease I (DNase I) was purified about 850,000-fold from human kidney using a rabbit anti-human urine DNase I antibody and sensitive DNase I activity assay. On SDS-PAGE, the purified kidney DNase I gave a single major band, and its molecular mass was estimated to be 38,000 Da. The activity of purified kidney DNase I was dependent on the presence of Mg2+ and Ca2+. G-Actin inhibited the activity, as did the anti-urine DNase I antibody. The properties of the kidney DNase I were the same as those of urine DNase I.Keywords
This publication has 0 references indexed in Scilit: