Different Protein-Lipid Interaction in Human Red Blood Cell Membrane of Rh Positive and Rh Negative Blood Compared with Rhnull

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Abstract
1-Anilino-naphthalene-8-sulfonate (ANS) fluorescence measurements revealed that human red blood cell membrane of the Rhnull type undergoes a transition at .apprx. 16.degree. C. Viscosity measurements of the extracted membrane lipids showed the usually observed transition at .apprx. 8.degree. C. Lower values of titratable SH groups were observed in Rhnull membrane using 5,5''-dithiobis-(2-nitro-benzoic-acid) (NBs2). Disulfide bonds in Rhnull membrane were .apprx. 3 times the value of the controls. Spin labeling experiments using 2-(3-carboxypropyl)-4,4 dimethyl-2-tridecyl 3-oxazolidinyl-oxyl were carried out with phospholipase A2 modified membranes. The mobile part of the spectra was significantly increased on the Rhnull membrane. In the presence of D-glucose, IR spectrometry showed a larger reduction of the intensity of the POO-band in Rhnull membrane. In contrast to controls, binding of the reagent diethylpyrocarbonate resulted in no significant changes of the Rhnull membrane as determined by ESR measurements. D-glucose transport activity was at the upper level of a group of Rh positive and Rh negative persons. The intensity of the polar protein-lipid interaction is apparently reduced Rhnull membrane.