Enzymatic and Immunological Approaches for the Quantitation and Confirmation of Ochratoxin A in Swine Kidneys
- 1 February 1997
- journal article
- Published by Elsevier in Journal of Food Protection
- Vol. 60 (2) , 172-176
- https://doi.org/10.4315/0362-028x-60.2.172
Abstract
Several techniques for the quantitation and confirmation of ochratoxin A (OA) in swine kidneys were examined. Naturally and artificially contaminated swine kidneys were analyzed for OA by conventional high-performance liquid chromatography (HPLC) analysis. Samples were additionally tested by enzyme-linked immunosorbent assay (ELISA) or treated with carboxypeptidase A followed by HPLC analysis (enzymatic method). Correlations (r values) between the conventional HPLC procedure and the ELISA, using artificially contaminated samples, were 0.88 and 0.81 (P < 0.05) respectively, while the corresponding values between the conventional HPLC procedure and the enzymatic method were 0.89 and 0.98 (P < 0.05). The ELISA gave a more direct estimation of OA contamination than the enzymatic procedure. The enzymatic method also had a reproducible tendency to underestimate or overestimate the amounts of OA in kidney. This was found to be dependent on the source of contamination, as artificially and naturally contaminated k...Keywords
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