5′-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation
- 1 May 1975
- journal article
- Published by Springer Nature in Nature
- Vol. 255 (5503) , 33-37
- https://doi.org/10.1038/255033a0
Abstract
Unmethylated reovirus and VSV mRNAs are specifically methylated to form 5'-terminal structures of the type, m-7-G(5')ppp(5')N by protein synthesising extracts prepared from wheat germ and mouse L cells. Reticulocyte mRNA also contains 5'-terminal m-7-G. MRNAs having 5'-terminal m-7-G stimulate protein synthesis in vitro. Removal of m-7-G by beta-elimination abolishes translation of the mRNAs.Keywords
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