Determination of 14C Distribution in Photosynthetic Serine and Phosphoglycerate from Grape Leaves
- 1 November 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 73 (3) , 579-581
- https://doi.org/10.1104/pp.73.3.579
Abstract
Serine and phosphoglyceric acid are the classical marker intermediates of photorespiration and reductive C assimilation in C3 plants. A new and fast method was presented for the determination of 14C distribution in these compounds by selective elimination of C-3 (NaIO4) or C-1 (ninhydrin/ceric sulfate). Reproducibility of the procedure was better than .+-.1% upon degradation of [U-14C]serine and [U-14C]glycerate standards. Determination of labeling patterns in (dephosphorylated) phosphoglycerate and serine, extracted from grape leaf discs after administration of 14CO2 for 1 min showed that 14C distribution in serine changes considerably with changing assimilatory conditions. Interpretations of data basing on unusual labeling patterns of serine must therefore be accepted with caution.This publication has 8 references indexed in Scilit:
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