Discrimination between major histocompatibility complex class II DQ and DR locus products in cattle
- 24 April 1995
- journal article
- Published by Wiley in Animal Genetics
- Vol. 26 (2) , 111-114
- https://doi.org/10.1111/j.1365-2052.1995.tb02643.x
Abstract
We have used a panel of anti-major histocompatibility complex (MHC) class II monoclonal antibodies (mAbs) and have assessed their specificity for the products of the individual bovine MHC (BoLA) class II subregions. The mAbs identified two distinct class II molecules by affinity purification and ELISA. Two-dimensional immunoblotting confirmed these data and NH2-terminal sequencing of the purified class II alpha chains of one member of each group identified the subregion specificity of the mAbs. The mAbs VPM36, TH22A and TH81A are specific for BoLA DQ, whereas VPM54, TH14B and J11 are specific for BoLA DR. SW73.2 reacts with both MHC subgroups of all cattle tested.Keywords
This publication has 21 references indexed in Scilit:
- POLYMORPHISM OF BOVINE MHC CLASS II GENES. JOINT REPORT OF THE FIFTH INTERNATIONAL BOVINE LYMPHOCYTE ANTIGEN (BOLA) WORKSHOP, INTERLAKEN, SWITZERLAND, 1 AUGUST 1992International Journal of Immunogenetics, 1994
- Improved discrimination of bovine class II DRβ‐chains polymorphisms using immunoblottingAnimal Genetics, 1994
- Patterns of major histocompatibility complex class II expression on T cell subsets in different immunological compartments 1. Expression on resting T cellsEuropean Journal of Immunology, 1993
- Isolation, characterization and evolution of ovine major histocompatibility complex class II DRA and DQA genesAnimal Genetics, 1993
- Nucleotide sequence and Northern analysis of a bovine major histocompatibility class II DRß‐like cDNAAnimal Genetics, 1991
- Monoclonal Antibodies to the Sheep Analogues of Human CD45 (Leucocyte Common Antigen), MHC Class I and CD5. Differential Expression after Lymphocyte Activation In VivoVeterinary Immunology and Immunopathology, 1990
- Isoelectric focusing of bovine major histocompatibility complex class II moleculesEuropean Journal of Immunology, 1989
- The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other speciesVeterinary Immunology and Immunopathology, 1987
- Genomic hybridization of bovine class II major histocompatibility genes: 2. Polymorphism of DR genes and linkage disequilibrium in the DQ‐DR regionAnimal Genetics, 1986
- Associations Between Major Histocompatibility Antigens and Susceptibility to DiseaseAnnual Review of Microbiology, 1979