Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli
- 1 April 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 134 (1) , 147-156
- https://doi.org/10.1128/jb.134.1.147-156.1978
Abstract
Thiomethyl-.beta.-galactoside (TMG) accumulation via the melibiose transport system was studied in lactose transport-negative strains of E. coli. TMG uptake by intact cells or membrane vesicles was markedly stimulated by Na+ or Li+ between pH 5.5-8. The Km for uptake of TMG was approximately 0.2 mM at an external Na+ concentration of 5 mM (pH 7). The .alpha.-galactosides, melibiose, methyl-.alpha.-galactoside and o-nitrophenyl-.alpha.-galactoside had a high affinity for this system but lactose, maltose and glucose had none. Evidence is presented for Li+-TMG or Na+-TMG cotransport.This publication has 47 references indexed in Scilit:
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