THE B REGION‐ASSOCIATED ANTIGENS AND MLR PHENOTYPES IN THE JAPANESE INBRED STRAINS OF RATS

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Abstract
Summary: Seven different alloantisera absorbed with red blood cells (RBC) from appropriate strains of rats detected a series of B cell alloantigenic specificities that could be divided into two groups, presumably coded for by at least two different closely‐linked loci in the rat major histocompatibility complex (MHC), RT1. The one locus had two allele codes for a broad specificity and the other locus codes for a unique specificity that was found only in the restricted strains of rats that shared the same mixed lymphocyte reaction (MLR) phenotype. RBC‐absorbed alloantisera were monitored against a panel of B cell fractions obtained from sixteen inbred strains. Two alloantisera, ACI anti‐W and W anti‐TO detected two broad specificities, into either of which all inbred strains tested were classified. Two broad RT1‐B region‐associated specificities were thus designated provisionally as Ba‐1.1 and ‐1.2. Another five alloantisera detected four respective specificities which have a narrower strain distribution. Sixteen inbred strains were classified into one of five specificities detected by W anti‐F344, F344 anti‐SDJ, WKA anti‐ACI, W anti‐BUF and ACI anti‐W absorbed with LEJ lymph node cells. Each specificity was designated provisionally as Ba‐2.1, ‐2.2, ‐2.4, ‐2.6, ‐2.7, respectively. A complete association of Ba‐2 specificities with MLR phenotype was observed. Antigenic specificities of Ba‐2.1, ‐2.2 and ‐2.4 were all classified in a group of Ba‐1.1 specificity, whereas Ba‐2.6 and ‐2.7 specificities were associated with Ba‐1.2 specificity. This relationship suggested a linkage disequilibrium between the two loci for the Ba antigens.