The identification of particulate matter in biological tissues and fluids

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Abstract
Sections, 1-2 .mu.m thick, of Araldite embedded [human synovial fluid] tissue were prepared to provide a link between optical microscopy and EM for the identification of crystals. This technique permits examination of individual particles by means of polarizing light microscopy, EM, X-ray energy spectroscopy and electron diffraction. It provides a reliable method for routine and research studies. It has led to the positive identification of individual crystals of calcium pyrophosphate dihydrate and hydroxyapatite in synovial fluids. The method can be applied to the identification of other particulate matter in a variety of biological specimens.