Complementation with ts Mutants of Herpes Simplex Virus

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Abstract
Tests to detect complementation between ts mutants of herpes simplex virus types 1 and 2 by infectious center and yield of virus assay were investigated. Progeny analysis of both intratypic and intertypic complementation showed a considerable proportion of recombinant or ts+ virus in the progeny; this was more marked in the infectious center tests. Virus of intermediate temperature-sensitivity was produced in intratypic and intertypic complementation. Reduction in the input multiplicity of 1 of the 2 mutants in the test to extremely low levels did not prevent complementation, suggesting that non-infectious particles probably contribute to complementation. Demonstration of virus DNA synthesis in mixedly-infected [baby hamster kidney, BHK-21] cells at the non-permissive temperature was used to detect complementation between DNA-negative mutants.