The Estimation and Extraction of Acetylcholine from Nervous Tissue of the American Cockroach

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Abstract
Although the nervous tissue of cockroaches contains higher titres of acetylcholine (ACh) than vertebrate nervous tissue on a wet-weight basis, it is necessary to use the nerve cords of a number of cockroaches for the assay of ACh by use of the rectus abdominis muscle of the frog. The number of nerve cords needed may be reduced by the elimination of any factor that tends to destroy ACh during the extraction procedure, or any that might reduce the threshold sensitivity of the muscle preparation to ACh. The results reported here show that trichloroacetic acid (TCA), when used in the method of Lewis and Smallman (1956) and Lewis and Fowler (1958) for the extraction of ACh from insect tissue, has a depressant effect on the rectus abdominis muscle of Rana pipiens.

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