Dissociation of nucleoprotein complexes by chaotropic salts
- 11 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (1) , 53-57
- https://doi.org/10.1016/0014-5793(83)80675-9
Abstract
The effect of various anions in destabilizing yeast nucleoprotein complexes followed the order F− < Cl− < Br− < ClO− 4 ⋍ Cl3CCOO−. Treatment of yeast nucleoproteins with 0.5 M NaClO4 resulted in removal of 80% of RNA. Based on the results, a simple method for effective separation of RNA from ribosomal particles is proposed and the mechanism of RNA dissociation by anions is also discussed.Keywords
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