Mutacin production in Streptococcus mutans genotypes isolated from caries‐affected and caries‐free individuals
- 20 December 2004
- journal article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 20 (1) , 20-24
- https://doi.org/10.1111/j.1399-302x.2005.00186.x
Abstract
Relationships between genetic diversity and mutacin production in Streptococcus mutans were evaluated in 319 clinical isolates from eight caries-affected and eight caries-free individuals. The isolates were submitted to mutacin typing and AP-PCR (arbitrarily primed polymerase chain reaction) assay. The mutacin production was detected for 12 Streptococcus sp. indicator strains. Results showed significant variations in the mutacin production profiles and the inhibitory spectra of both groups. A possible association was seen between mutacin activity and the distinct patterns of Streptococcus sp. colonization in the two groups. Genotyping by AP-PCR using the primers OPA-02 and OPA-13 revealed 101 distinct genotypes against 48 phenotypes identified by mutacin typing. No correlation was observed between the inhibitory spectra of mutacin and genotypic similarities based on AP-PCR analyses. According to our results, strains of the same S. mutans genotype showed different mutacin profiles, suggesting a high degree of interstrain diversity. In conclusion, mutacin production seems to be of clinical importance in the colonization of S. mutans and is highly diversified in the S. mutans species.Keywords
This publication has 28 references indexed in Scilit:
- Genotypic diversity and virulence traits of Streptococcus mutans in caries-free and caries-active individualsJournal of Medical Microbiology, 2004
- Determination of mutacin activity and detection of mutA genes in Streptococcus mutans genotypes from caries-free and caries-active childrenOral Microbiology and Immunology, 2003
- The Group I Strain of Streptococcus mutans , UA140, Produces Both the Lantibiotic Mutacin I and a Nonlantibiotic Bacteriocin, Mutacin IVApplied and Environmental Microbiology, 2001
- Similarity of Bacteriocin Activity Profiles of Mutans Streptococci within the Family When the Children Acquire the Strains After the age of 5Caries Research, 2000
- Typing of mutans streptococci by arbitrarily primed polymerase chain reactionArchives of Oral Biology, 1996
- Oral colonization by more than one clonal type of mutans streptococcus in children with nursing-bottle dental cariesArchives of Oral Biology, 1996
- Comparison of the Initial Streptococcal Microflora on Dental Enamel in Caries-Active and in Caries-Inactive IndividualsCaries Research, 1990
- Mouth-to-mouth transmission of the bacterium Streptococcus mutans between mother and childArchives of Oral Biology, 1985
- Bacteriocin of a Group B Streptococcus: Partial Purification and CharacterizationAntimicrobial Agents and Chemotherapy, 1975
- Inhibitory Spectrum of a Bacteriocinlike Substance (Mutacin) Produced by Some Strains of Streptococcus mutansJournal of Dental Research, 1975